cell-cycle analysis module watson-pragmatic model Search Results


v10  (Becton Dickinson)
90
Becton Dickinson v10
V10, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell-cycle+analysis+module+watson-pragmatic+model/pmc09799308-241-12-14?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
v10 - by Bioz Stars, 2026-07
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cell cycle watson  (Becton Dickinson)
90
Becton Dickinson cell cycle watson
Cell Cycle Watson, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell-cycle+analysis+module+watson-pragmatic+model/pmc07554040-177-21-21?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
cell cycle watson - by Bioz Stars, 2026-07
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facscanto  (Becton Dickinson)
90
Becton Dickinson facscanto
A, CP-31398 stimulated the generation of ROS, which was blocked by pre-treating cells with NAC. Cells were cultured in medium containing CP-31398 (20 μg/ml) for 0, 12 and 24 h in the absence or presence of 5 mM NAC; B, Pretreatment of CsA blocked CP-31398–induced generation of ROS. ROS generation was measured by using the ROS-detecting fluorescent dye, DCF-DA on a flow cytometry. The corresponding linear diagram of <t>FACScan</t> is also shown (n=3, mean ± S.D; **P < 0.01). Cells were treated with CP-31398 (20μg/ml) for 15min. Each value represents mean ± S.D. of three independent experiments (n=3, *P < 0.01).
Facscanto, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell-cycle+analysis+module+watson-pragmatic+model/pmc02922473-63-11-11?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
facscanto - by Bioz Stars, 2026-07
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facscalibur  (Becton Dickinson)
90
Becton Dickinson facscalibur
A, CP-31398 stimulated the generation of ROS, which was blocked by pre-treating cells with NAC. Cells were cultured in medium containing CP-31398 (20 μg/ml) for 0, 12 and 24 h in the absence or presence of 5 mM NAC; B, Pretreatment of CsA blocked CP-31398–induced generation of ROS. ROS generation was measured by using the ROS-detecting fluorescent dye, DCF-DA on a flow cytometry. The corresponding linear diagram of <t>FACScan</t> is also shown (n=3, mean ± S.D; **P < 0.01). Cells were treated with CP-31398 (20μg/ml) for 15min. Each value represents mean ± S.D. of three independent experiments (n=3, *P < 0.01).
Facscalibur, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell-cycle+analysis+module+watson-pragmatic+model/pmc08517100-383-12-13?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
facscalibur - by Bioz Stars, 2026-07
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lsrii instrument  (Becton Dickinson)
90
Becton Dickinson lsrii instrument
A, CP-31398 stimulated the generation of ROS, which was blocked by pre-treating cells with NAC. Cells were cultured in medium containing CP-31398 (20 μg/ml) for 0, 12 and 24 h in the absence or presence of 5 mM NAC; B, Pretreatment of CsA blocked CP-31398–induced generation of ROS. ROS generation was measured by using the ROS-detecting fluorescent dye, DCF-DA on a flow cytometry. The corresponding linear diagram of <t>FACScan</t> is also shown (n=3, mean ± S.D; **P < 0.01). Cells were treated with CP-31398 (20μg/ml) for 15min. Each value represents mean ± S.D. of three independent experiments (n=3, *P < 0.01).
Lsrii Instrument, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell-cycle+analysis+module+watson-pragmatic+model/pm28752867-226-6-7?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
lsrii instrument - by Bioz Stars, 2026-07
90/100 stars
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lsr fortessa cell analyzer  (Becton Dickinson)
90
Becton Dickinson lsr fortessa cell analyzer
A, CP-31398 stimulated the generation of ROS, which was blocked by pre-treating cells with NAC. Cells were cultured in medium containing CP-31398 (20 μg/ml) for 0, 12 and 24 h in the absence or presence of 5 mM NAC; B, Pretreatment of CsA blocked CP-31398–induced generation of ROS. ROS generation was measured by using the ROS-detecting fluorescent dye, DCF-DA on a flow cytometry. The corresponding linear diagram of <t>FACScan</t> is also shown (n=3, mean ± S.D; **P < 0.01). Cells were treated with CP-31398 (20μg/ml) for 15min. Each value represents mean ± S.D. of three independent experiments (n=3, *P < 0.01).
Lsr Fortessa Cell Analyzer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell-cycle+analysis+module+watson-pragmatic+model/pmc07554040-177-10-14?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
lsr fortessa cell analyzer - by Bioz Stars, 2026-07
90/100 stars
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lsrii  (Becton Dickinson)
90
Becton Dickinson lsrii
A, CP-31398 stimulated the generation of ROS, which was blocked by pre-treating cells with NAC. Cells were cultured in medium containing CP-31398 (20 μg/ml) for 0, 12 and 24 h in the absence or presence of 5 mM NAC; B, Pretreatment of CsA blocked CP-31398–induced generation of ROS. ROS generation was measured by using the ROS-detecting fluorescent dye, DCF-DA on a flow cytometry. The corresponding linear diagram of <t>FACScan</t> is also shown (n=3, mean ± S.D; **P < 0.01). Cells were treated with CP-31398 (20μg/ml) for 15min. Each value represents mean ± S.D. of three independent experiments (n=3, *P < 0.01).
Lsrii, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell-cycle+analysis+module+watson-pragmatic+model/10__1039_slash_c7dt03229c-278-6-7?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
lsrii - by Bioz Stars, 2026-07
90/100 stars
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univariate cell cycle platform  (Becton Dickinson)
90
Becton Dickinson univariate cell cycle platform
A, CP-31398 stimulated the generation of ROS, which was blocked by pre-treating cells with NAC. Cells were cultured in medium containing CP-31398 (20 μg/ml) for 0, 12 and 24 h in the absence or presence of 5 mM NAC; B, Pretreatment of CsA blocked CP-31398–induced generation of ROS. ROS generation was measured by using the ROS-detecting fluorescent dye, DCF-DA on a flow cytometry. The corresponding linear diagram of <t>FACScan</t> is also shown (n=3, mean ± S.D; **P < 0.01). Cells were treated with CP-31398 (20μg/ml) for 15min. Each value represents mean ± S.D. of three independent experiments (n=3, *P < 0.01).
Univariate Cell Cycle Platform, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell-cycle+analysis+module+watson-pragmatic+model/pm37235189__im3c00007_si_001-280-8-17?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
univariate cell cycle platform - by Bioz Stars, 2026-07
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v. 10.8.1  (Becton Dickinson)
90
Becton Dickinson v. 10.8.1
LOC441461 suppresses cell proliferation by promoting G1/S transition in human gastric cancer cells. ( A ) Knockdown of LOC441461 by transfection with si- LOC441461 or a scrambled negative control (N.C) in MKN74 cells. The proliferation ability of N.C- and si- LOC441461 -transfected cells was examined using the CCK-8 solution at every 24 h. ( B ) The cell cycle progression of N.C- and si- LOC441461 -transfected cells was examined using flow cytometry with the PI/RNase solution. Each cell cycle phase was analyzed with FlowJo v. 10.8.1. Graph of each quantified phase. ( C ) MKN74 and SNU216 cells were transfected with N.C or si- LOC441461 and stained with 0.01% crystal violet solution after 14 days. ( D ) Graph of colony formation ability after elution with 33% acetic acid. All experiments were performed in triplicate, and data are expressed as the mean ± standard deviation (* p < 0.05, ** p < 0.01; Student’s t -test).
V. 10.8.1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell-cycle+analysis+module+watson-pragmatic+model/pmc08909665-95-6-9?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
v. 10.8.1 - by Bioz Stars, 2026-07
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facscanto ii flow cytometer  (Becton Dickinson)
90
Becton Dickinson facscanto ii flow cytometer
LOC441461 suppresses cell proliferation by promoting G1/S transition in human gastric cancer cells. ( A ) Knockdown of LOC441461 by transfection with si- LOC441461 or a scrambled negative control (N.C) in MKN74 cells. The proliferation ability of N.C- and si- LOC441461 -transfected cells was examined using the CCK-8 solution at every 24 h. ( B ) The cell cycle progression of N.C- and si- LOC441461 -transfected cells was examined using flow cytometry with the PI/RNase solution. Each cell cycle phase was analyzed with FlowJo v. 10.8.1. Graph of each quantified phase. ( C ) MKN74 and SNU216 cells were transfected with N.C or si- LOC441461 and stained with 0.01% crystal violet solution after 14 days. ( D ) Graph of colony formation ability after elution with 33% acetic acid. All experiments were performed in triplicate, and data are expressed as the mean ± standard deviation (* p < 0.05, ** p < 0.01; Student’s t -test).
Facscanto Ii Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell-cycle+analysis+module+watson-pragmatic+model/pmc09161009-271-13-17?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
facscanto ii flow cytometer - by Bioz Stars, 2026-07
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facscanto ii instrument  (Becton Dickinson)
90
Becton Dickinson facscanto ii instrument
LOC441461 suppresses cell proliferation by promoting G1/S transition in human gastric cancer cells. ( A ) Knockdown of LOC441461 by transfection with si- LOC441461 or a scrambled negative control (N.C) in MKN74 cells. The proliferation ability of N.C- and si- LOC441461 -transfected cells was examined using the CCK-8 solution at every 24 h. ( B ) The cell cycle progression of N.C- and si- LOC441461 -transfected cells was examined using flow cytometry with the PI/RNase solution. Each cell cycle phase was analyzed with FlowJo v. 10.8.1. Graph of each quantified phase. ( C ) MKN74 and SNU216 cells were transfected with N.C or si- LOC441461 and stained with 0.01% crystal violet solution after 14 days. ( D ) Graph of colony formation ability after elution with 33% acetic acid. All experiments were performed in triplicate, and data are expressed as the mean ± standard deviation (* p < 0.05, ** p < 0.01; Student’s t -test).
Facscanto Ii Instrument, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell-cycle+analysis+module+watson-pragmatic+model/pmc06661975__oc9b00266_si_001-236-7-10?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
facscanto ii instrument - by Bioz Stars, 2026-07
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Image Search Results


A, CP-31398 stimulated the generation of ROS, which was blocked by pre-treating cells with NAC. Cells were cultured in medium containing CP-31398 (20 μg/ml) for 0, 12 and 24 h in the absence or presence of 5 mM NAC; B, Pretreatment of CsA blocked CP-31398–induced generation of ROS. ROS generation was measured by using the ROS-detecting fluorescent dye, DCF-DA on a flow cytometry. The corresponding linear diagram of FACScan is also shown (n=3, mean ± S.D; **P < 0.01). Cells were treated with CP-31398 (20μg/ml) for 15min. Each value represents mean ± S.D. of three independent experiments (n=3, *P < 0.01).

Journal:

Article Title: Targeting wild-type and mutant p53 with small molecule CP-31398 blocks the growth of rhabdomyosarcoma by inducing ROS-dependent apoptosis

doi: 10.1158/0008-5472.CAN-10-0942

Figure Lengend Snippet: A, CP-31398 stimulated the generation of ROS, which was blocked by pre-treating cells with NAC. Cells were cultured in medium containing CP-31398 (20 μg/ml) for 0, 12 and 24 h in the absence or presence of 5 mM NAC; B, Pretreatment of CsA blocked CP-31398–induced generation of ROS. ROS generation was measured by using the ROS-detecting fluorescent dye, DCF-DA on a flow cytometry. The corresponding linear diagram of FACScan is also shown (n=3, mean ± S.D; **P < 0.01). Cells were treated with CP-31398 (20μg/ml) for 15min. Each value represents mean ± S.D. of three independent experiments (n=3, *P < 0.01).

Article Snippet: Flow cytometric analysis of cell cycle Flow cytometry was done using Becton Dickinson FACSCan and cell cycle was analyzed using FowJo (8.8.6) Watson pragmatic analysis software.

Techniques: Cell Culture, Flow Cytometry

LOC441461 suppresses cell proliferation by promoting G1/S transition in human gastric cancer cells. ( A ) Knockdown of LOC441461 by transfection with si- LOC441461 or a scrambled negative control (N.C) in MKN74 cells. The proliferation ability of N.C- and si- LOC441461 -transfected cells was examined using the CCK-8 solution at every 24 h. ( B ) The cell cycle progression of N.C- and si- LOC441461 -transfected cells was examined using flow cytometry with the PI/RNase solution. Each cell cycle phase was analyzed with FlowJo v. 10.8.1. Graph of each quantified phase. ( C ) MKN74 and SNU216 cells were transfected with N.C or si- LOC441461 and stained with 0.01% crystal violet solution after 14 days. ( D ) Graph of colony formation ability after elution with 33% acetic acid. All experiments were performed in triplicate, and data are expressed as the mean ± standard deviation (* p < 0.05, ** p < 0.01; Student’s t -test).

Journal: Cancers

Article Title: Downregulation of LOC441461 Promotes Cell Growth and Motility in Human Gastric Cancer

doi: 10.3390/cancers14051149

Figure Lengend Snippet: LOC441461 suppresses cell proliferation by promoting G1/S transition in human gastric cancer cells. ( A ) Knockdown of LOC441461 by transfection with si- LOC441461 or a scrambled negative control (N.C) in MKN74 cells. The proliferation ability of N.C- and si- LOC441461 -transfected cells was examined using the CCK-8 solution at every 24 h. ( B ) The cell cycle progression of N.C- and si- LOC441461 -transfected cells was examined using flow cytometry with the PI/RNase solution. Each cell cycle phase was analyzed with FlowJo v. 10.8.1. Graph of each quantified phase. ( C ) MKN74 and SNU216 cells were transfected with N.C or si- LOC441461 and stained with 0.01% crystal violet solution after 14 days. ( D ) Graph of colony formation ability after elution with 33% acetic acid. All experiments were performed in triplicate, and data are expressed as the mean ± standard deviation (* p < 0.05, ** p < 0.01; Student’s t -test).

Article Snippet: Cell cycle progression was analyzed using FlowJo v. 10.8.1 (FlowJo LLC, Ashland, OR, USA) with the Watson (Pragmatic) model.

Techniques: Transfection, Negative Control, CCK-8 Assay, Flow Cytometry, Staining, Standard Deviation